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Molecular cloning of canine RCAS1 cDNA
Yasuhiko Okamura1, Zhiyong Ma, Tanvir S Khatlani
1Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi, Japan.
Abstract:
Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a novel cancer cell-surface antigen, strongly expressed in invasive cancers. RCAS1 inhibited the in vitro growth of immunocytes, and induced apoptotic cell death. The cloning of canine RCAS1 cDNA was carried out and identified from the mammary gland tumor of a dog. A canine RCAS1 cDNA of 864 bp in length has an open reading frame of 642 bp nucleotides encoding a protein of 213 deduced amino acids. The predicted amino acid sequence of canine RCAS1 showed 96.2% and 96.7% homologies with those of human and mouse RCAS1 respectively. Canine RCAS1 has an N-terminal transmembrane segment and a coiled-coil structure in the C-terminal protein, which are highly conserved in mouse and human RCAS1.
Insights
Researchers identified canine Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) from mammary tumors. This novel cancer antigen is highly conserved across species and inhibits immune cell growth, inducing apoptosis.
Area of Science:
- Immunology
- Oncology
- Molecular Biology
Background:
- Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a novel cancer cell-surface antigen.
- RCAS1 is strongly expressed in invasive cancers and inhibits immunocyte growth, inducing apoptosis.
Purpose of the Study:
- To clone and identify canine RCAS1 cDNA.
- To analyze the structural and functional conservation of canine RCAS1.
Main Methods:
- Cloning of canine RCAS1 cDNA from a mammary gland tumor.
- Sequence analysis of canine RCAS1 cDNA and protein.
Main Results:
- Canine RCAS1 cDNA is 864 bp with a 642 bp open reading frame encoding 213 amino acids.
- Canine RCAS1 protein shares 96.2% and 96.7% homology with human and mouse RCAS1, respectively.
- Key structural features, including a transmembrane segment and coiled-coil structure, are conserved.
Conclusions:
- Canine RCAS1 is a highly conserved protein with functional similarities to human and mouse RCAS1.
- The identification of canine RCAS1 provides a valuable model for studying cancer immunology and antigen function.