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Updated: Jul 11, 2026
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
Published on: March 2, 2017
In vitro regulation of beta1 and beta3 integrin subunits in endometrial epithelial cells from normal endometrium
Emilia Pierro1, Francesca Minici, Ornella Alesiani
1Unità Operativa di Ginecologia ed Ostetricia, Ospedale G.B. Grassi, Roma, Italy
Objective:
To evaluate the effect of prostaglandin E2 (PGE2) and interleukin-1beta (IL-1beta) on integrin expression. DESIGN Cultures of endometrial epithelial cells from normal endometrium.
Setting:
All endometrial specimens were obtained from the Obstetrics and gynecology Department of the Catholic University, Rome, Italy.
Patients:
Four patients were normal menstrual cycles undergoing operative laparoscopy for non-endometrial problems.
Intervention:
Endometrial samples were collected by uterine courettings.
Main Outcome Measure:
Immunocytochemistry for beta1 and beta3 integrin subunits.
Results:
PGE2 clearly enhances both beta1 and beta3 integrin subunit expression. IL-1beta seem to slightly increase only beta3 subunit expression.
Conclusions:
In light of the critical role played by eicosanoids in endometrial differentiation, we suggest that PGE2 is also involved in local paracrine regulation of integrin expression.
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