Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially defined microenvironmental niches are associated with clinical outcome and tumor ecosystem diversity in head and neck cancer.

Med (New York, N.Y.)·2026
Same author

Association of Intratumoral Microbiota with Prognosis in Head and Neck Squamous Cell Carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Viscoelastic properties of tumor spheroids revealed by a microfluidic compression device and a modified power law model.

Soft matter·2026
Same author

A microfluidic rheometer for tumor mechanics and invasion studies.

bioRxiv : the preprint server for biology·2025
Same author

A microfluidic rheometer for tumor mechanics and invasion studies.

Lab on a chip·2025
Same author

Viscoelastic properties of tumor spheroids revealed by a microfluidic compression device and a modified power law model.

ArXiv·2025

Related Experiment Video

Updated: Jan 13, 2026

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

9.2K

Local signaling by the EGF receptor.

Stephan J Kempiak1, Shu-Chin Yip, Jonathan M Backer

  • 1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, New York, NY 10461, USA.

The Journal of Cell Biology
|September 4, 2003
PubMed
Summary

Cell signaling spatial scales guide cell orientation in chemotaxis. Epidermal growth factor (EGF) triggers localized actin polymerization but widespread ERK activation, revealing distinct signaling ranges.

More Related Videos

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

7.0K
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

9.1K

Related Experiment Videos

Last Updated: Jan 13, 2026

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

9.2K
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
05:57

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

7.0K
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

9.1K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Cellular orientation during chemotaxis relies on signaling cascades operating at different spatial scales.
  • Understanding these scales is crucial for deciphering cell movement and response mechanisms.

Purpose of the Study:

  • To investigate the spatial dynamics of Epidermal Growth Factor Receptor (EGFR) signaling.
  • To determine how localized stimulation affects downstream pathways like actin polymerization and ERK activation.

Main Methods:

  • Utilized biotinylated EGF bound to streptavidin-coupled magnetic beads for localized cell stimulation.
  • Studied cells overexpressing the EGF receptor to analyze signaling responses.
  • Assessed actin polymerization and ERK activation localization and dependency on specific signaling molecules.

Main Results:

  • Epidermal growth factor (EGF)-induced actin polymerization remained localized to the stimulation site, even with EGFR overexpression.
  • Conversely, EGF-induced ERK activation exhibited a widespread, cell-body-spanning distribution.
  • Localized actin polymerization was found to be independent of PI3-kinase and Rho activity, but dependent on Arp2/3 complex and cofilin.

Conclusions:

  • EGFR signaling operates at distinct spatial scales, with localized actin dynamics and widespread ERK activation.
  • These findings support chemotaxis models integrating both short-range and long-range signaling mechanisms.
  • The differential spatial control of signaling pathways provides insights into precise cell orientation during chemotaxis.