[Fluorescence quenching method for the determination of trace copper with DBH-PF]

A fluorescence quenching method for the determination of trace Cu(II) was developed. The method was based on the fluorescence quenching effect of DBH-PF and Triton X-100 due to the formation of a non-fluorescent ternary complex in pH 6.2-8.2 KH2PO4-Na2HPO4 medium. The excitation and emission wavelengths are 530 and 560 nm, respectively. The linear range is 0-80 micrograms.L-1 and the detection limit is 0.20 microgram.L-1 for Cu(II). The method has been used to determine trace amounts of Cu(II) in hair, tea and water. The recovery is 98%-104%, and the relative standard deviation is less than 6.9% with satisfactory results.