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Updated: Sep 20, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
A new epitope on swine CD5 molecule detected by monoclonal antibody 5F12/9
N Doménech1, B Alvarez, R Bullido
1Unidad de Investigación, Complejo Hospitalario Juan Canalejo, 15006 A Coruña, Spain. ndomenech@canalejo.org
Abstract:
This paper describes the production and characterization of a monoclonal antibody (MAb), 5F12/9, that recognizes a new epitope on porcine CD5. Conformation of its CD5 specificity was obtained by means of sequential immunoprecipitation and Western blot experiments in combination with anti-CD5 MAb 1H6/8, whereas cross-blocking experiments with both MAbs showed that they reacted with different epitopes.
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