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Related Concept Videos

Folliculogenesis01:20

Folliculogenesis

Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...

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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
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In vitro growth of preantral follicles isolated from cryopreserved ovine ovarian tissue.

Sandra Cecconi1, Giulia Capacchietti, Valentina Russo

  • 1Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell'Aquila, 67100, L'Aquila, Italy.

Biology of Reproduction
|September 5, 2003
PubMed
Summary

Cryopreservation of sheep preantral follicles using dimethyl sulfoxide (DMSO) or ethylene glycol (EG) impacted their in vitro development. While follicles grew, cryopreservation affected oocyte chromatin configuration and metabolic cooperativity.

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Published on: September 27, 2022

Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Ovarian Tissue Cryopreservation

Background:

  • Sheep preantral follicles are crucial for reproductive potential.
  • Cryopreservation offers a method for preserving ovarian tissue.
  • Understanding cryoprotectant effects is vital for successful fertility preservation.

Purpose of the Study:

  • To compare the in vitro development of sheep preantral follicles after cryopreservation using different cryoprotectants.
  • To evaluate the impact of slow-freezing with dimethyl sulfoxide (DMSO) or ethylene glycol (EG) on follicle survival, growth, and oocyte quality.

Main Methods:

  • Sheep ovarian cortex was cryopreserved using slow-freezing with DMSO or EG.
  • Mechanically isolated follicles were cultured in vitro for 10 days.
  • Histological analysis, oocyte-cumulus cell complex (OCC) assessment, estradiol measurement, and [3H]uridine uptake were performed.

Main Results:

  • Both DMSO and EG cryopreservation led to follicle degeneration post-thawing, with higher rates in DMSO.
  • Despite growth and antrum formation, DMSO-treated follicles yielded fewer healthy OCCs and lower estradiol levels.
  • Cryopreserved OCCs exhibited reduced metabolic cooperativity, and a lower percentage of oocytes showed germinal vesicle stage-arrest with rimmed chromatin.

Conclusions:

  • Cryopreservation of sheep preantral follicles allows in vitro growth but negatively impacts gap junctional permeability and oocyte chromatin configuration.
  • Ethylene glycol may be a preferable cryoprotectant over DMSO for sheep preantral follicle cryopreservation.
  • The study successfully differentiated cryoprotectant effects from direct cellular damage during cryopreservation.