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Related Experiment Videos

Parallel preparative high-performance liquid chromatography with on-line molecular mass characterization.

Christine Edwards1, Jianbo Liu, Thomas J Smith

  • 1Biotage, Inc., A Dyax Corp Company, P.O. Box 8006, Charlottesville, VA 22906, USA. cedwards@biotage.com

Rapid Communications in Mass Spectrometry : RCM
|September 5, 2003
PubMed
Summary

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High-throughput chemistry purification bottlenecks are reduced by integrating parallel preparative HPLC with UV-guided fraction collection and online mass spectrometry (MS) for improved compound identification.

Area of Science:

  • Chemical synthesis and purification
  • Pharmaceutical lead discovery
  • Analytical chemistry

Background:

  • High-throughput chemistry (HTC) is crucial in pharmaceutical lead discovery.
  • Purification, particularly high-performance liquid chromatography (HPLC), is a bottleneck in HTC.
  • Post-purification analysis complicates the identification of target compounds.

Purpose of the Study:

  • To address the purification bottleneck in HTC.
  • To improve the efficiency of compound identification after purification.
  • To integrate online mass spectrometry with preparative HPLC.

Main Methods:

  • Utilized parallel preparative HPLC with UV-guided fraction collection.
  • Interfaced the Biotage Parallex system with a MUX interface.

Related Experiment Videos

  • Connected the system to a single quadrupole mass spectrometer for online MS characterization.

    • Main Results:

    • Successfully combined robust UV-guided fractionation with online MS characterization.
    • Demonstrated a method to reduce complexity in post-purification analysis.
    • Enabled simultaneous purification and characterization of compounds.

    Conclusions:

    • The integration of preparative HPLC and online MS streamlines the lead discovery process.
    • This approach enhances the efficiency of identifying purified compounds.
    • It offers a solution to the purification and analysis bottleneck in HTC.