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Studies on kinetic model of vitamin C two-step fermentation process
Chinese Journal of Biotechnology
|January 1, 1992
Summary
This study analyzes the bioconversion of L-sorbose to 2-Keto-L-gulonic acid using a fermentation model. The developed kinetic model accurately predicts experimental data, validating its reliability for this bioprocess.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Chemical Synthesis
Background:
- L-sorbose is a precursor for vitamin C synthesis.
- Efficient bioconversion is crucial for industrial production.
- Understanding fermentation kinetics aids process optimization.
Purpose of the Study:
- To analyze the bioconversion of L-sorbose to 2-Keto-L-gulonic acid.
- To develop and validate a kinetic model for the fermentation process.
- To investigate the fermentation mechanism with simplifications.
Main Methods:
- Development of a simplified fermentation model.
- Application of nonlinear optimization techniques.
- Utilizing the Runge-Kutta method for parameter estimation.
Main Results:
- A reliable kinetic model was established for the fermentation process.
- Computer simulations based on the model showed strong agreement with experimental data.
- The growth factor assumption aided in analyzing the bioconversion.
Conclusions:
- The developed kinetic model is reliable for describing the L-sorbose to 2-Keto-L-gulonic acid bioconversion.
- The study provides a validated model for optimizing this key step in vitamin C production.
- Further research can build upon this model for enhanced bioprocess control.