Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Labile DNA sequences in flax identified by combined sample representational difference analysis (csRDA).

Thomas J Oh1, Christopher A Cullis

  • 1NovoMark Technologies, 2915 Southington Road, Shaker Heights, OH 44120, USA.

Plant Molecular Biology
|September 6, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EMS Derived Wheat Mutant BIG8-1 (<i>Triticum aestivum</i> L.)-A New Drought Tolerant Mutant Wheat Line.

International journal of molecular sciences·2021
Same author

Wheat Line "RYNO3936" Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery.

Frontiers in plant science·2020
Same author

Plant Vacuolar Processing Enzymes.

Frontiers in plant science·2019
Same author

Expression of a Small Ubiquitin-Like Modifier Protease Increases Drought Tolerance in Wheat (<i>Triticum aestivum</i> L.).

Frontiers in plant science·2019
Same author

Cysteine proteases and wheat (Triticum aestivum L) under drought: A still greatly unexplored association.

Plant, cell & environment·2017
Same author

Agroinfiltration contributes to VP1 recombinant protein degradation.

Bioengineered·2016

Flax genotrophs exhibit stable phenotypes, unlike the parental line, indicating genetic changes associated with environmental plasticity. Representational difference analysis identified DNA differences, suggesting structural alterations in some genotrophs.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Flax (Linum usitatissimum) exhibits phenotypic plasticity influenced by environmental factors.
  • Genotrophs, derived from the inbred flax variety Stormont Cirrus (Pl), display stable phenotypes across environments.
  • These genotrophs differ from the parental line, notably in their lack of environmentally induced changes.

Purpose of the Study:

  • To identify genetic loci controlling phenotypic plasticity in flax.
  • To investigate DNA differences between the parental line and stable genotrophs.
  • To understand the molecular basis of environmentally induced changes in flax.

Main Methods:

  • Representational difference analysis (RDA) was employed to compare DNA from Pl and genotrophs.

Related Experiment Videos

  • Both individual and combined sample RDA (csRDA) were performed, along with contrary RDA.
  • Difference clones were identified, and PCR and Southern blot analyses were used for characterization.

    • Main Results:

    • Several difference clones were isolated, with some showing polymorphisms between Pl and genotrophs.
    • Clone 163-13 polymorphism was confirmed by Southern blot.
    • Four characterized clones appear associated with structural DNA changes in genotrophs.

    Conclusions:

    • Genotrophs possess stable phenotypes, suggesting genetic mechanisms underlying their environmental stability.
    • RDA successfully identified DNA differences potentially linked to plasticity.
    • Structural DNA alterations may contribute to the stable phenotypes observed in flax genotrophs.