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Updated: Sep 20, 2026
Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
Published on: August 9, 2024
Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in
Jian-Hui Liu1, Yong-Ming Bao, Ji-Jun Song
1Department of Biochemical Engineering, Dalian University of Technology, Dalian 116024, China.
Aim:
To explore the effect of Codonopsis pilosula (Franch) Nannf total alkaloids (DSA) on differentiation induced by nerve growth factor (NGF) in PC12 cells.
Methods:
After culturing PC12 cells with DSA in the presence or absence of NGF, neurite outgrowth in PC12 cells and correlated protein kinases were assayed.
Results:
DSA alone did not exhibit neuritogenic activity, but caused a significant enhancement of NGF (2 microg/L)-induced neurite outgrowth in PC12 cells, and increased the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, this enhancing effect was completely blocked by a specific MAPK kinase inhibitor, PD98059.
Conclusion:
DSA enhanced the NGF-induced neurite outgrowth in PC12 cells by amplifying an up-stream step of the MAPK-dependent signaling pathway.
