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Related Experiment Videos

Cell phenotype analysis using a cell fluid-based microchip with high sensitivity and accurate quantitation.

Chichen Michael Chien1, Jing-Long Cheng, Wen-Teish Chang

  • 1Institute of Life Science, National Tsing Hua University, 300 Hsinchu, Taiwan.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|September 6, 2003
PubMed
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This study introduces a rapid cell fluid chip assay for analyzing cell phenotypes using minimal cell samples. The assay effectively quantifies changes in natural killer (NK) cells after Ganoderma lucidum extract treatment.

Area of Science:

  • Cell biology
  • Immunology
  • Biotechnology

Background:

  • Conventional flow cytometry requires large cell numbers for phenotype analysis.
  • There is a need for rapid, high-throughput methods for cell characterization.
  • Small cell population analysis is challenging with existing techniques.

Purpose of the Study:

  • To evaluate a cell fluid chip-based cytometric assay for rapid phenotype characterization.
  • To assess the assay's utility in analyzing small cell populations and screening herbal extracts.
  • To investigate the effect of Ganoderma lucidum extract on umbilical cord blood (UCB) mononuclear cell subtypes.

Main Methods:

  • A novel cell fluid chip-based fluorescent cytometric assay was developed and run on a bioanalyzer.
  • The assay determined cell surface marker expression on various cell samples, analyzing six samples per chip.

Related Experiment Videos

  • The method was validated against conventional flow cytometry and applied to screen herbal extracts for immunomodulatory effects.

    • Main Results:

    • The chip-based assay demonstrated good quantitative agreement with conventional flow cytometry.
    • It required significantly fewer cells (<200 cells/sample) compared to conventional methods (10^5 cells/sample).
    • Treatment with Ganoderma lucidum extract (F3) significantly increased CD56+ natural killer (NK) cells in UCB mononuclear cells from 1.1% to 3.2%.

    Conclusions:

    • The cell fluid chip assay provides a fast and efficient method for cell phenotype characterization, especially for small cell populations.
    • This method is suitable for screening herbal extracts and assessing their impact on immune cell activity.
    • Ganoderma lucidum extract quantitatively influences NK cell activity, highlighting its potential immunomodulatory properties.