Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ribosome assembly in eukaryotes.

Micheline Fromont-Racine1, Bruno Senger, Cosmin Saveanu

  • 1Genetique des Interactions Macromoleculaires, Institut Pasteur, 75724 Cedex 15, Paris, France. mfromont@pasteur.fr

Gene
|September 6, 2003
PubMed
Summary

Ribosome biogenesis is a complex process involving over 170 factors in eukaryotes, primarily focusing on RNA maturation, export, and assembly. Recent proteomic studies reveal a dynamic pathway for ribosome assembly.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of ergosteryl-aspartate synthase reveals how an entrapped tRNA is used like a prosthetic swinging arm in the synthesis of aminoacylated sterols.

Nature communications·2026
Same author

Nonsense-mediated mRNA decay factors target short poly(A)-tailed mRNAs lacking a premature termination codon.

Nature communications·2026
Same author

Biochemical Insights Into the Conserved Interactions of NMD Factors From Budding Yeast to Humans.

Journal of molecular biology·2026
Same author

A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes.

eLife·2025
Same author

Oligomer-based functions of mitochondrial porin.

Nature communications·2025
Same author

RNA anchoring of Upf1 facilitates recruitment of Dcp2 in the NMD decapping complex.

Nucleic acids research·2025

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Ribosome synthesis is a fundamental cellular process essential for protein production.
  • This complex process occurs across multiple cellular compartments, including the nucleolus, nucleoplasm, and cytoplasm.
  • Over 170 protein factors are currently known to be involved in eukaryotic ribosome biogenesis, with more being identified.

Purpose of the Study:

  • To summarize the current understanding of ribosome biogenesis in eukaryotic cells.
  • To highlight the roles of various factors, particularly in RNA maturation, export, and assembly.
  • To present insights into the eukaryotic ribosome assembly pathway.

Main Methods:

  • Analysis of protein composition of yeast ribosomal subunit precursors.

Related Experiment Videos

  • Proteomic approaches to identify and characterize factors involved in ribosome biogenesis.
  • Review of recent advances in understanding ribosome export and assembly.

    • Main Results:

    • Identification of over 170 protein factors participating in ribosome biogenesis.
    • Majority of identified factors are involved in RNA maturation (nucleotide modification and processing).
    • Proteomic data suggests a dynamic ribosome assembly pathway in eukaryotes.

    Conclusions:

    • Ribosome synthesis is a highly coordinated process involving numerous factors and multiple cellular locations.
    • Recent proteomic studies have shed light on the dynamic nature of ribosome assembly and export.
    • Further research continues to expand our knowledge of this essential cellular machinery.