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Published on: April 28, 2015
Use of water-dispersible Fe(2)O(3) nanoparticles with narrow size distributions in isolating avidin
Jinda Fan1, Junge Lu, Ruisong Xu
1Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL 62901-4409, USA.
Abstract:
Synthetic approaches that vigorously control the microstructures of water-dispersible gamma-Fe(2)O(3) nanoparticles such as size and size uniformity are of importance to the potential biological applications of these nanomaterials. In the present paper, water-dispersible gamma-Fe(2)O(3) nanocrystals with narrow size distributions (bipy-Fe(2)O(3)) were prepared via a site-exchange reaction. These particular materials are superparamagnetic and stable within a wide range of pH. Introduction of the biotin functionality onto the surfaces of bipy-Fe(2)O(3) enabled the affinity isolation of the protein avidin from its incubation solution magnetically with 96% efficiency.

