Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inhibitors of hepatic microsomal triacylglycerol hydrolase decrease very low density lipoprotein secretion.

Dean Gilham1, Samuel Ho, Mehdi Rasouli

  • 1Department of Cell Biology, CIHR Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, Canada T6G 2S2.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|September 6, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Building a foundation to promote Women's health research in Dubai. Study protocol for a project to investigate the feasibility of establishing a dedicated Women's health biobank.

Frontiers in global women's health·2026
Same author

Growth Plate Injuries: Advances and Future Directions in Regenerative Medicine.

Annals of biomedical engineering·2025
Same author

Harnessing MSC Immunomodulation in Orthopedics: Clinical Insights for Comorbidities.

Stem cell reviews and reports·2025
Same author

CES1 Increases Hepatic Triacylglycerol Synthesis Through Activation of PPARγ, LXR and SREBP1c.

Cells·2025
Same author

Alterations in phosphatidylethanolamine metabolism impacts hepatocellular lipid storage, energy homeostasis, and proliferation.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2025
Same author

FITM2 deficiency results in ER lipid accumulation, ER stress, and reduced apolipoprotein B lipidation and VLDL triglyceride secretion in vitro and in mouse liver.

Molecular metabolism·2024

Inhibiting triacylglycerol hydrolase significantly reduced the secretion of very low-density lipoprotein (VLDL) particles, including apolipoprotein B-100 (apoB-100). This suggests triacylglycerol hydrolase is a potential drug target for lowering blood lipids.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiovascular Disease Research

Background:

  • Elevated circulating triacylglycerol (TG)-rich very low-density lipoprotein (VLDL) and apolipoprotein B-100 (apoB-100) are independent risk factors for coronary artery disease.
  • Triacylglycerol hydrolase (TGH) mobilizes cytoplasmic TG stores, but its role in VLDL assembly is unclear.

Purpose of the Study:

  • To investigate the role of TGH in providing core lipids for VLDL assembly.
  • To determine the effect of inhibiting TGH activity on lipid and apoB synthesis and secretion.

Main Methods:

  • Primary rat hepatocytes and hepatoma cells transfected with TGH cDNA were used.
  • Lipase activity was inhibited to analyze lipid and apoB synthesis and secretion.
  • Secreted lipoproteins and apolipoproteins were analyzed.

Related Experiment Videos

Main Results:

  • Inhibition of lipolysis dramatically decreased TG secretion.
  • Secretion of cholesteryl ester and phosphatidylcholine was also substantially reduced.
  • ApoB-100 secretion was more sensitive to lipase inhibition than apoB-48 secretion.

Conclusions:

  • Intracellular TG hydrolysis significantly decreases apoB-100 secretion.
  • TGH may be a viable pharmacological target for reducing plasma lipid levels.
  • Preferential lipidation of apoB-100 was observed in TGH-transfected cells.