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Chemically defined media for growth of Haemophilus influenzae strains
Hannah N Coleman1, Dayle A Daines, Justin Jarisch
1Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
Journal of Clinical Microbiology
|September 6, 2003
Abstract:
A chemically defined medium that supports the growth of both encapsulated and nontypeable Haemophilus influenzae strains in broth to densities that are >/= 10(9) CFU/ml or on agar plates is described. The mean generation time of a panel of clinical isolates was comparable to that in rich, chemically undefined media (brain-heart infusion broth supplemented with heme and beta-NAD).