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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens
Akihiro Yoshida1, Nao Suzuki, Yoshio Nakano
1Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
Journal of Clinical Microbiology
|September 6, 2003
Abstract:
A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.