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Multiplex PCRs for identification of necrotoxigenic Escherichia coli
Sigrid Van Bost1, Etienne Jacquemin, Eric Oswald
1University of Liège, Faculty of Veterinary Medicine, Laboratory of Bacteriology, Sart-Tilman B43a, B-4000 Liège, Belgium. svanbost@ulg.ac.be
Journal of Clinical Microbiology
|September 6, 2003
Abstract:
Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necrotoxigenic E. coli strains isolated from different animal species.