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Updated: Sep 20, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
[Study on the reaction between 3-amino-6-dimethylamino-2-methlphenazine hydrochloride and deoxyribonucleic acid]
1Department of Material and Engineering, Southwest University of Science and Technology, Mianyang 621002, China.
Abstract:
The interaction of deoxyribonucleic acid (DNA) and 3-amino-6-dimethylamino-2-methlphenazine hydrochloride (ZR) was investigated by UV spectrophotometric method in solution (pH 7.4). When DNA was added into ZR solution, the red color was observed, which indicated formation of the DNA-ZR complex. The maximum absorption of the complex was 520 nm with the apparent molar absorptivity of epsilon = 1.5 x 10(6) mol-1.L.cm-1. The maximum absorption of the complex was shifted 70 nm than with ZR. The maximum binding number is n = 303. The basic reaction conditions were investigated. It is found that sodium chloride concentration of the solution has significant effect on the sensitivity of DNA-ZR complex. The Scatchard model is appropriate in the treatment of data obtained here.
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