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Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
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[Determination of deoxyribonucleic acid with neutral red by resonance light scattering method]

H Y Xiang1, X M Chen, S Q Li

  • 1Institute of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan 411105, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|September 10, 2003
PubMed

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...