Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Loading the multilayer dextran sulfate/protamine microsized capsules with peroxidase.

Nadezda G Balabushevich1, Olga P Tiourina, Dmitry V Volodkin

  • 1Max-Planck Institute of Colloids and Interfaces, Golm/Potsdam, 14476 Germany.

Biomacromolecules
|September 10, 2003
PubMed
Summary

Stable biodegradable polyelectrolyte capsules were created using layer-by-layer assembly. These capsules effectively encapsulate horseradish peroxidase, maintaining high activity for 12 months.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Porogen-Mediated Barrier Control in Multilayered Drug-Eluting Antibacterial Films: Comparative Evaluation of PEG, PVP, and PEOx.

Pharmaceutics·2026
Same author

Magnetoelectric biodegradable uniform composite microactuators for biomedical applications.

Journal of materials chemistry. B·2026
Same author

Retraction: Triple-responsive inorganic-organic hybrid microcapsules as a biocompatible smart platform for the delivery of small molecules.

Journal of materials chemistry. B·2026
Same author

Minimally Invasive Endovascular Administration for Targeted PLGA Nanoparticles Delivery to Brain, Salivary Glands, Kidney and Lower Limbs.

Pharmaceutics·2026
Same author

Light-directed reprogramming of tumor-associated macrophages via STING agonist delivery.

Biomaterials advances·2025
Same author

The olfactory epithelium as a gateway for bloodborne nanoparticles to the central nervous system.

Biomaterials science·2025

Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Biodegradable polyelectrolytes offer promising materials for encapsulation.
  • Layer-by-layer (LbL) assembly is a versatile technique for creating complex structures.
  • Melamine formaldehyde (MF) microcores provide a template for capsule formation.

Purpose of the Study:

  • To develop stable, biodegradable polyelectrolyte capsules.
  • To investigate the pH-dependent swelling and loading capacity of these capsules.
  • To assess the stability and activity of encapsulated horseradish peroxidase (HRP).

Main Methods:

  • LbL assembly of dextran sulfate and protamine on MF microcores.
  • Core decomposition at low pH to form hollow capsules.

Related Experiment Videos

  • Encapsulation of HRP and quantification using Raman confocal spectroscopy.
  • pH-dependent swelling studies and stability assessments.

    • Main Results:

    • Stable capsules with diameters ranging from 8.0 to 10 microm were produced.
    • Capsule size and HRP loading capacity increased with pH.
    • Encapsulated HRP retained 57% activity and remained stable for 12 months.
    • MF residues and polymers formed a gel-like matrix embedding the HRP.

    Conclusions:

    • LbL assembly provides a robust method for creating biodegradable polyelectrolyte capsules.
    • The pH-responsive nature of the capsules allows for tunable loading and size.
    • The developed capsules are suitable for stable enzyme encapsulation with long-term activity preservation.