Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cutting Edge: Multiple autoimmune pathways in kd/kd mice.

Wayne W Hancock1, Tsai-Lung Tsai, Michael P Madaio

  • 1Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia and the University of Philadelphia School of Medicine, Philadelphia, PA 19104, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|September 10, 2003
PubMed
Summary

This study investigated kidney disease (kd) progression in congenic mice. Results indicate the initial kidney defect is intrinsic, with diverse immune cell compositions contributing to disease severity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential effects of HDAC8 targeting on Foxp3+ Tregs and effector T cells promote antitumor immunity.

JCI insight·2025
Same author

CAR T cells targeting CCR4 selectively deplete human Tregs ex vivo and in vivo.

Blood advances·2025
Same author

Class IIa HDACs forced degradation allows resensitization of oxaliplatin-resistant FBXW7-mutated colorectal cancer.

Molecular oncology·2025
Same author

MEF2D regulates T-cell function via CD70-CD27 signaling and promotes immune evasion in hepatocellular carcinoma.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

A descriptive survey evaluating the implementation and outcomes of a training session highlighting concepts in antimicrobial management and harm reduction for hospital-based clinicians treating persons who use drugs.

Therapeutic advances in infectious disease·2025
Same author

New Approaches to Old Techniques in Cell Handling for Microscopy.

Cells·2025

Area of Science:

  • Immunology
  • Nephrology
  • Genetics

Background:

  • The kidney disease (kd) mutation was introduced onto a C57BL/6 (B6) background, creating the B6.kd congenic strain.
  • This strain was subsequently crossed with mice carrying targeted mutations in various immune-related genes.

Purpose of the Study:

  • To determine the role of specific immune components in the pathogenesis of kidney disease.
  • To investigate whether the kidney disease mutation's effects are intrinsic to the kidney or dependent on specific immune responses.

Main Methods:

  • Congenic strain creation (B6.kd) and genetic crosses with CD4, CD8, CD28, IL-2, Rag-1, ICAM-1, and beta(2)-microglobulin knockout mice.
  • Phenotypic analysis of kidney disease severity and frequency in resulting double mutants.
  • Immunohistological examination of infiltrating immune cells within kidney lesions.

Related Experiment Videos

Main Results:

  • Kidney disease developed similarly in B6.kd mice and most double mutants, except for a slightly reduced frequency in CD28(-/-) kd/kd mice.
  • Immunohistology revealed a primary infiltration of macrophages in B6.kd and most double mutants, with variable T and NK cell presence.
  • In Rag-1(-/-) kd/kd mice, infiltrating cells were composed of roughly 50% macrophages and 50% NK cells.

Conclusions:

  • The primary kidney defect caused by the kd mutation appears to be intrinsic to the kidney.
  • The subsequent immune response contributing to kidney disease can involve diverse cellular compositions, highlighting immune system plasticity.