Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modeling TCR signaling complex formation in positive selection.

Katherine J Hare1, Judit Pongracz, Eric J Jenkinson

  • 1Department of Anatomy, Medical Research Council Centre for Immune Regulation, University of Birmingham, Birmingham, United Kingdom. K.J.Hare@bham.ac.uk

Journal of Immunology (Baltimore, Md. : 1950)
|September 10, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thymus health is a predictor of lifelong well-being and immunotherapy effectiveness.

Nature·2026
Same author

When immunity multitasks: type 2 pathways power thymus regeneration.

Immunology letters·2026
Same author

Claudin 1-mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance.

The Journal of experimental medicine·2026
Same author

Regulatory T cell stability determines the efficiency of bile duct regeneration during cholangitis.

Cell reports·2025
Same author

PD-1 receptor deficiency enhances CD30<sup>+</sup> T<sub>reg</sub> cell function in melanoma.

Nature immunology·2025
Same author

Establishment of a microwell-array-based miniaturized thymic organoid model suitable for high-throughput applications.

Cell reports·2025

T cell receptor (TCR) signaling strength in the thymus dictates T cell fate. CD80 costimulation alters TCR signaling by affecting lipid rafts, influencing T cell maturation or apoptosis.

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • T cell receptor (TCR) signaling in the thymus determines T cell fate through positive or negative selection.
  • The precise mechanisms by which TCR ligation at the CD4(+)8(+) stage leads to distinct cell fates remain unclear.
  • Signal strength is hypothesized to be a critical factor in dictating the outcome of TCR signaling.

Purpose of the Study:

  • To characterize the formation of active signaling complexes during positive selection in thymocytes.
  • To investigate the role of costimulatory molecule CD80 in modulating TCR signaling and thymocyte development.
  • To explore the relationship between lipid raft dynamics and T cell fate decisions.

Main Methods:

  • Characterization of signaling complex formation in thymocytes interacting with thymic epithelial cells.

Related Experiment Videos

  • Analysis of cell surface and intracellular molecule redistribution during T cell activation.
  • Assessment of GM1-containing lipid raft accumulation and polarization.
  • Enforced expression of CD80 on thymic epithelium to study its effects on thymocyte development.

    • Main Results:

    • Active signaling complexes form during positive selection, involving molecular redistribution but not GM1 lipid raft accumulation.
    • Enforced CD80 expression on thymic epithelium induced GM1 polarization in thymocytes.
    • CD80 costimulation led to reduced positive selection and increased apoptosis in CD4(+)8(+) thymocytes.
    • Differential lipid raft recruitment appears to be influenced by CD80 costimulation.

    Conclusions:

    • TCR signaling strength, modulated by CD80 costimulation, influences thymocyte developmental fate.
    • CD80 costimulation impacts TCR signaling outcomes through differential lipid raft recruitment.
    • Understanding these mechanisms is crucial for comprehending T cell maturation and selection processes in the thymus.