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Both aging and chronic fluoxetine increase S100B content in the mouse hippocampus
Mustafa Akhisaroglu1, Radmila Manev, Ezgi Akhisaroglu
1The Psychiatric Institute, Department of Psychiatry, University of Illinois at Chicago, 1601 West Taylor Street, MC912, Chicago, IL 60612, USA.
Neuroreport
|September 10, 2003
Summary
Aging increases hippocampal S100B protein, a cytokine linked to neurotrophic activity. Fluoxetine treatment further elevated S100B in aged mice, indicating its efficacy is not impaired by aging.
Area of Science:
- Neuroscience
- Molecular Biology
- Aging Research
Background:
- S100B is a cytokine with neurotrophic and neurite-extending properties.
- S100B is implicated in antidepressant mechanisms and aging-associated disorders like Alzheimer's disease.
- Brain S100B levels increase with age in humans.
Purpose of the Study:
- To investigate the effect of aging on hippocampal S100B protein levels.
- To determine if fluoxetine treatment affects hippocampal S100B in aged mice.
- To assess the impact of aging on fluoxetine's action on hippocampal S100B.
Main Methods:
- Quantitative Western immunoblotting was used to assay hippocampal S100B protein content.
- An immunoassay kit was employed for S100B protein quantification.
- Experiments were conducted on young (2-month-old) and old (24-month-old) mice, with some old mice receiving 2 weeks of fluoxetine treatment.
Main Results:
- Hippocampal S100B protein content was significantly higher in old mice compared to young mice.
- Fluoxetine treatment of old mice led to a further increase in hippocampal S100B levels.
- These findings suggest that aging does not hinder fluoxetine's effect on hippocampal S100B.
Conclusions:
- Aging is associated with increased hippocampal S100B protein.
- Fluoxetine effectively increases hippocampal S100B in aged mice.
- The antidepressant fluoxetine's mechanism involving S100B is preserved in aging.