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CD84 expression on human hematopoietic progenitor cells.

Matthias Zaiss1, Christian Hirtreiter, Michael Rehli

  • 1Abteilung für Hämatologie und Internistische Onkologie and Institut für Pathologie, University of Regensburg, Regensburg, Germany. matthias.zaiss@klinik.uni-regensburg.de

Experimental Hematology
|September 10, 2003
PubMed
Summary
This summary is machine-generated.

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CD84 is found on most blood cells and progenitor cells. Its expression on CD34+ cells indicates commitment to erythroid lineages and reduced proliferation.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • CD84, a member of the immunoglobulin receptor superfamily (CD2 subgroup), is involved in T and NK cell activation.
  • Previous studies identified CD84 expression on mononuclear blood cells and platelets.
  • The expression pattern on immature and mature hematopoietic cells remained largely uncharacterized.

Purpose of the Study:

  • To investigate the expression of CD84 on human immature CD34+ and mature hematopoietic cells.
  • To determine the role of CD84 in hematopoietic progenitor cell differentiation and proliferation.

Main Methods:

  • Flow cytometry was employed for expression analysis of CD84 on various hematopoietic cells.
  • Colony-forming assays and long-term cultures assessed the differentiation potential of CD84+ progenitor cells.

Related Experiment Videos

  • RT-PCR was utilized to analyze CD84 mRNA isoforms.

    • Main Results:

    • CD84 is expressed on granulocytes, monocytes, macrophages, B cells, and some T cells.
    • CD84 was detected on 64% of peripheral blood CD34+ progenitor cells and 30.5% of bone marrow CD34+ cells.
    • CD34+CD84+ cells predominantly formed erythroid colonies, with reduced myeloid potential, and showed decreased long-term culture-initiating capacity compared to CD34+CD84- cells.

    Conclusions:

    • CD84 is present on cells across nearly all hematopoietic lineages and on CD34+ hematopoietic progenitor cells.
    • Increasing CD84 expression on CD34+ cells correlates with decreased proliferative potential.
    • CD84 may serve as a marker for committed hematopoietic progenitor cells, particularly those differentiating into erythroid lineages.