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Related Experiment Videos

DNA supercoiling in apoptotic chromatin.

Cristina D'Arrigo1, Paola Barboro, Michele Mormino

  • 1Istituto per lo Studio delle Macromolecole, Sezione di Genova, Via De Marini, 6 16146 Genoa, Italy.

Biochemical and Biophysical Research Communications
|September 10, 2003
PubMed
Summary

Histone deacetylation during apoptosis does not cause chromatin condensation. Instead, chromatin condenses into a network of supercoiled fibers, similar to changes induced by chromatin remodeling factors.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Epigenetics

Background:

  • Previous research indicated chromatin structural changes and increased unacetylated histones H3 and H4 during rat thymocyte apoptosis.
  • The relationship between histone deacetylation and chromatin condensation remained unclear.

Purpose of the Study:

  • To investigate the role of H3 and H4 deacetylation in chromatin condensation during apoptosis.
  • To elucidate the ultrastructural and topological organization of condensed chromatin.

Main Methods:

  • Ultrastructural observations of apoptotic rat thymocytes.
  • Ethidium bromide relaxation experiments to analyze chromatin topology.
  • Analysis of chromatin fiber organization and DNA supercoiling.

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Main Results:

  • H3 and H4 deacetylation was found to be unrelated to chromatin condensation.
  • Condensed chromatin exhibits a tightly woven network of negatively supercoiled 11 nm fibers.
  • Apoptotic chromatin loops showed nucleosome oligomers and supercoiled DNA, resembling alterations induced by SWI/SNF and RSC remodeling factors.

Conclusions:

  • Histone deacetylation is not the cause of chromatin condensation in thymocyte apoptosis.
  • Apoptotic chromatin condensation involves the formation of supercoiled fiber networks.
  • The observed chromatin modifications suggest a potential role for chromatin remodeling factors in apoptosis-induced structural changes.