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Published on: October 9, 2016
Splicing isoform of SYT-SSX fusion protein accelerates transcriptional activity and cell proliferation
Yuki Morimoto1, Mamoru Ouchida, Toshifumi Ozaki
1Science of Functional Recovery and Reconstruction, Department of Molecular Genetics, Graduate School of Medicine and Dentistry, Okayama University, Shikata-cho 2-5-1, Okayama 700-8558, Japan.
Abstract:
The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.
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