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Related Experiment Videos

Skin prick testing with standardized extracts from 3 different manufacturers. A comparative randomized study.

N H Nielsen1, A Dirksen, H Mosbech

  • 1Allergy Clinic, Frederiksborg County Hospital, Copenhagen, Denmark.

Allergologia Et Immunopathologia
|November 1, 1992
PubMed
Summary

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Comparing allergen prick test kits from three manufacturers revealed variations in reactivity. While overall positive reaction frequencies were similar, specific allergen responses differed, impacting clinical panel selection for allergy diagnosis.

Area of Science:

  • Allergy and Immunology
  • Dermatology
  • Clinical Diagnostics

Background:

  • Skin prick testing is a common method for diagnosing allergic diseases.
  • Standardization of allergen extracts and testing methods can vary between manufacturers.
  • Understanding inter-manufacturer variability is crucial for accurate allergy diagnosis.

Purpose of the Study:

  • To compare the skin reactivity of allergen prick test panels from three different manufacturers.
  • To assess the consistency and variability of allergen responses in routine clinical use.

Main Methods:

  • Two hundred consecutive patients underwent skin prick testing with ten common aero-allergens.
  • Allergen panels were sourced from three manufacturers: ALK (Soluprick SQ), DHS (Alphatest), and PHA (Phazet).

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  • Methods for allergen standardization, potency declaration, and lancet use differed across manufacturers.

    • Main Results:

    • The coefficient of variation for allergen extracts was 0.31 for PHA, 0.13 for ALK, and 0.18 for DHS.
    • Overall frequencies of positive patient reactions to allergens were generally similar across the three panels.
    • The DHS panel showed fewer positive reactions to Timothy pollen, dog dander, and Dermatophagoides pteronyssinus compared to others.

    Conclusions:

    • Significant differences in allergen extract variability and specific allergen reactivity exist between manufacturers.
    • Physicians should be aware of the allergenic activity differences in routine panels for accurate allergy diagnosis.
    • Manufacturer-specific performance may influence the interpretation of skin prick test results.