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Updated: Sep 20, 2026
Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
Published on: May 18, 2014
[Allogenic transplantation studies on human fetal pancreatic nestin positive cells]
Hai Xia Huang1, Xin Zhi Huang, Xin Yu Chen
1Department of Cell Biology, Shanghai Second Medical University, Shanghai 200025.
Human fetal pancreatic nestin positive cells cultured in vitro could self assemble to form islet-like cell clusters(ICC). They are multipotential and are capable of generating insulin-producing cells. To investigate their biological character and physiological function in vivo, ICCs were implanted into NOD-Scid mice subcapsularly. The results showed that: (1) Neovascularizations were observed in implant sites. (2) Blood glucose levels of diabetic mice were reduced markedly after implantation of ICCs. (3) ICCs in non-diabetic mice proliferated abnormally and infiltrated into renal parenchyma with many cell structures formed.
Human fetal pancreatic nestin positive cells cultured in vitro could self assemble to form islet-like cell clusters(ICC). They are multipotential and are capable of generating insulin-producing cells. To investigate their biological character and physiological function in vivo, ICCs were implanted into NOD-Scid mice subcapsularly. The results showed that: (1) Neovascularizations were observed in implant sites. (2) Blood glucose levels of diabetic mice were reduced markedly after implantation of ICCs. (3) ICCs in non-diabetic mice proliferated abnormally and infiltrated into renal parenchyma with many cell structures formed.
