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Expression of CD23 by human bone marrow stromal cells
C Fourcade1, M Arock, S Ktorza
1Laboratoire d'Immunologie, CHU Pitié-Salpêtrière, Paris, France.
European Cytokine Network
|November 1, 1992
Summary
CD23 antigen expression on stromal cells supports hematopoietic cell development in bone marrow cultures. Blocking CD23 significantly reduced hematopoietic cell numbers and colony formation, highlighting its regulatory role.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- CD23 is a surface antigen found on various human hematopoietic cells.
- CD23 exhibits diverse biological functions.
- The role of CD23 in bone marrow stromal cells is not fully understood.
Purpose of the Study:
- To investigate CD23 expression in human bone marrow (BM) and BM-derived stromal cells.
- To determine the function of CD23 in long-term bone marrow cultures (LTBMC).
Main Methods:
- Assaying CD23 expression on freshly isolated BM cells and LTBMC-derived stromal cells.
- Measuring CD23 mRNA and soluble CD23 secretion.
- Utilizing neutralizing anti-CD23 monoclonal antibody (mAb) in LTBMC.
Main Results:
- Freshly isolated BM cells showed low CD23 expression.
- A subset of LTBMC-derived stromal cells expressed high levels of CD23 mRNA and secreted soluble CD23.
- Inhibition of CD23 function with anti-CD23 mAb led to a significant decrease in hematopoietic cell numbers and CFU-GM recovery in LTBMC.
Conclusions:
- CD23 expression plays a role in the function of bone marrow stromal cells.
- CD23 regulates human hematopoietic cell development.
- Soluble CD23 may be involved in modulating hematopoietic cell growth.