Use of naloxone challenge to predict sexual performance of young rams

The possibility of developing a hormone-based test to predict libido was evaluated using the response of LH and testosterone to naloxone. This test has been used to identify sexually active and inactive mature rams during the breeding season. The objective of this study was to determine whether the blood test could be used to detect differences in sexual activity of early postpubertal (29 +/- 0.1 wk) rams during the breeding season in November and again at 70 +/- 0.1 wk of age in August before the next breeding season. Rams were classed as sexually active or inactive using serving capacity tests (8 30-min observation periods to record sexual behaviors [mounts and ejaculations] of each ram individually exposed to three ewes in estrus) after the naloxone challenges. Naloxone (0.75 mg/kg of BW) was injected i.v. into 38 white-faced crossbred, 16 Polypay, and 49 Targhee rams. Blood samples were collected at 15-min intervals for 1 h before and 2 h after naloxone to measure LH and testosterone. Separate mixed-model analyses for repeated measures were used to analyze data for the same rams at 29 and 70 wk of age. Logistic regression procedures were used to model probabilities that rams were correctly predicted to be sexually active. A breed-type x sexual activity x time interaction for LH was observed (P < 0.05) after naloxone injection in 29-wk-old rams. At 70 wk of age, a breed-type x time interaction was detected (P < 0.001) for LH response to naloxone, but LH did not differ by sexual activity. At 29 wk of age, a breed-type x time interaction for testosterone response after naloxone was detected (P < 0.001), and at 70 wk of age, a sexual activity x time interaction was detected (P < 0.05) for testosterone after naloxone. Sexually active and inactive rams were not predicted accurately at 29 wk of age and were predicted 69 and 29% of the time for sexually active and inactive rams, respectively, at 70 wk of age. In conclusion, breed type at 29 and 70 wk of age can influence the naloxone challenge test, but the test cannot be used to discriminate between sexually active and inactive rams at 29 wk of age during the breeding season or at 70 wk of age immediately before the breeding season.