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Binding of extracellular matrix molecules by probiotic bacteria
I Styriak1, R Nemcová, Y-H Chang
1Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia. styriak@saske.sk
Letters in Applied Microbiology
|September 13, 2003
Summary
Selected bacterial strains demonstrate strong extracellular matrix (ECM) binding, comparable to commercial probiotics. This suggests potential for wider use in animal feed and probiotic preparations for enhanced gut colonization.
Area of Science:
- Microbiology
- Probiotics
- Animal Science
Background:
- Extracellular matrix (ECM) binding is crucial for probiotic bacteria to colonize the host intestine.
- Evaluating the ECM binding capabilities of novel bacterial isolates is essential for identifying new probiotic candidates.
Purpose of the Study:
- To compare the extracellular matrix (ECM) and mucin binding of selected bacterial isolates with probiotic features against commercially available probiotic bacteria.
- To assess the potential of novel bacterial strains for use in animal probiotic applications based on their ECM binding abilities.
Main Methods:
- Bacterial strains were tested for their ability to bind immobilized ECM molecules, including porcine mucin, fetuin, bovine fibrinogen, porcine fibronectin, and bovine lactoferrin.
- Binding affinities were quantified using microtitre plates and latex bead assays.
Main Results:
- All tested probiotic strains exhibited binding to porcine mucin, with significant inter-strain variations.
- Binding to fetuin was generally weak across all strains.
- Strong binding to bovine fibrinogen and porcine fibronectin was observed in specific strains, including some commonly used commercial probiotics and novel isolates.
- Bovine lactoferrin showed higher binding affinity compared to transferrins.
Conclusions:
- Certain animal probiotic strains, specifically L. casei L.c. and Lactobacillus sp. 2I3, show comparable extracellular matrix (ECM) binding capabilities to commercially established probiotic strains.
- These findings suggest that these animal strains hold promise for broader application in fermented feed and probiotic formulations for animals, potentially improving intestinal colonization.