Related Experiment Video
Updated: Sep 20, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot
Heping Dai1, Hong Gao, Xinyan Zhao
1Institute of Hydrobiology, Academy of Chinese Sciences, Wuchan, Hubei 430072, Wuhan, People's Republic of China. hpdai@ihb.ac.cn
Abstract:
An antibody phage display library against White Spot Syndrome Virus (WSSV) was constructed. After four rounds of panning against WSSV, 192 out of 480 clones displayed WSSV binding activity. One of the positive clones, designated A1, had relatively higher activity specifically binding to WSSV. A1-soluble, single-chain fragment variable (scFv) antibody has an affinity constant (K(aff)) of 2.02+/-0.42x10(9) M(-1). Dot blot assays showed that A1-soluble scFv could detect WSSV directly from shrimp hemolymph after 24-h feeding infection by WSSV. A1 scFv has potential for the development of a cheap, simple and sensitive diagnostic kit for WSSV in the field.
