Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TRAIL regulates normal erythroid maturation through an ERK-dependent pathway.

Paola Secchiero1, Elisabetta Melloni, Markku Heikinheimo

  • 1Dept of Morphology and Embryology, Human Anatomy Section, University of Ferrara, Via Fossato di Mortara 66, 44100 Ferrara, Italy. secchier@mail.umbi.umd.edu

Blood
|September 13, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Macrophage Polarization and Spatial Architecture Characterize the Tumor Immune Microenvironment and Prognosis in Hepatoblastoma.

The American journal of pathology·2026
Same author

Exploratory Volatilome Profiling of Inflammation in Skin Fibroblasts: A Proof-of-Concept Study.

International journal of molecular sciences·2026
Same author

GATA4-targeted compounds induce apoptosis and diminish viability of hepatoblastoma cells.

PloS one·2026
Same author

Biological Evaluation and SAR Exploration of Bile Acid-Dihydroartemisinin Hybrids as Potential Anticancer Agents for Colorectal Cancer.

Biomolecules·2026
Same author

Anticancer activity of MDM2 inhibition in 2D and bioprinted 3D retinoblastoma cell models.

Frontiers in pharmacology·2025
Same author

Targeting Intestinal Permeability for Graft-versus-Host Disease Treatment: A Therapeutic Perspective with Defibrotide.

Journal of experimental pharmacology·2025

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) impacts human erythropoiesis by inducing apoptosis and inhibiting erythroblast maturation via the ERK1/2 pathway. Inhibiting ERK1/2 rescues erythroid differentiation.

Area of Science:

  • Hematology
  • Cell Biology
  • Immunology

Background:

  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a key mediator of apoptosis.
  • Erythropoiesis, the process of red blood cell formation, is crucial for oxygen transport.
  • Understanding TRAIL's role in erythropoiesis can reveal new therapeutic targets.

Purpose of the Study:

  • To investigate the biologic activity of TRAIL on human erythropoiesis.
  • To identify TRAIL receptors and intracellular pathways involved in its effects on erythroid cells.
  • To determine the impact of TRAIL on erythroid differentiation and maturation.

Main Methods:

  • Generation of glycophorin A (GPA)+ erythroid cells from human cord blood (CB) CD34+ progenitor cells in serum-free culture.

Related Experiment Videos

  • Analysis of TRAIL receptor expression (TRAIL-R1, -R2, -R3, -R4) during erythroid differentiation.
  • Treatment with recombinant TRAIL and pathway inhibitors (z-VAD-fmk, PD98059, SB203580) to assess effects on apoptosis and differentiation.

    • Main Results:

    • TRAIL-R1 and TRAIL-R2 were expressed on erythroid cells, while TRAIL-R3 and -R4 were not detected.
    • Recombinant TRAIL induced apoptosis and inhibited the maturation of erythroblasts.
    • TRAIL significantly stimulated the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway, which mediated its negative effects on erythroid maturation.

    Conclusions:

    • TRAIL exerts biologic activity on human erythropoiesis, primarily through TRAIL-R2.
    • The ERK1/2 pathway is a critical mediator of TRAIL's inhibitory effects on erythroid differentiation.
    • Targeting the ERK1/2 pathway may offer a strategy to modulate TRAIL-induced inhibition of erythropoiesis.