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Multilineage gene expression in human bone marrow stromal cells as evidenced by single-cell microarray analysis.
Beerelli Seshi1, Sanjay Kumar, Debra King
1Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, 12902 Magnolia Drive, Tampa, FL 33612-9497, USA. BSeshi@rei.edu
Blood Cells, Molecules & Diseases
|September 16, 2003
Summary
Bone marrow stromal cells are a single cell type expressing multiple phenotypes, not multiple cell types. This finding supports a common progenitor for mesenchymal and hematopoietic cells.
Area of Science:
- Cell Biology
- Hematology
- Developmental Biology
Background:
- Bone marrow stromal cells are crucial for hematopoietic stem cell development.
- The heterogeneity of mesenchymal stromal cell cultures has been debated.
Purpose of the Study:
- To determine if bone marrow stromal cell cultures contain multiple mesenchymal stromal cell types or a single type with diverse phenotypes.
- To investigate the differentiation potential and molecular characteristics of individual bone marrow stromal cells.
Main Methods:
- Single-cell transcriptome analysis of bone marrow stromal cells cultured in the Dexter system.
- Analysis of gene expression profiles associated with various cell lineages and hematopoietic markers.
Main Results:
- Individual bone marrow stromal cells express transcripts for osteoblast, fibroblast, muscle, and adipocyte differentiation.
- Single stromal cells also exhibit transcripts characteristic of epithelial, endothelial, and neural/glial cells.
- Expression of hematopoietic markers (CD45, CD19, CD10, CD79a) and proto-oncogenes was observed in stromal cells, suggesting a common progenitor with B-lymphocytes.
Conclusions:
- Bone marrow stromal cells are relatively homogeneous, exhibiting a broad differentiation capacity.
- The findings support the concept of progenitor cells expressing genes for multiple potential lineage paths.
- A common progenitor for nonhematopoietic mesenchymal cells and hematopoietic B-lymphocytes is suggested.