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Bacteriological study of root canals associated with periapical abscesses
Ezilmara Leonor Rolim de Sousa1, Caio Cezar Randi Ferraz, Brenda Paula Figueiredo de Almeida Gomes
1University of Campinas, Brazil. cferraz@fop.unicamp.br
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
|September 16, 2003
Summary
Microorganisms in root canals with periapical abscesses were identified, revealing Gram-positive anaerobes as predominant. Peptostreptococcus prevotii and Fusobacterium necrophorum showed susceptibility to tested antibiotics, offering insights for endodontic treatment.
Area of Science:
- Microbiology
- Endodontics
- Infectious Diseases
Background:
- Periapical abscesses are often associated with complex microbial communities in root canals.
- Identifying these microorganisms is crucial for effective treatment strategies.
Purpose of the Study:
- To identify microorganisms present in root canals of teeth with periapical abscesses.
- To determine the antimicrobial susceptibility of key bacterial species, Peptostreptococcus prevotii and Fusobacterium necrophorum.
Main Methods:
- Microbiological sampling of 30 root canals using sterile paper points.
- Identification of concomitant microorganisms using established microbiological techniques.
- Antimicrobial susceptibility testing of P. prevotii and F. necrophorum via E test method.
Main Results:
- A total of 117 bacterial strains were isolated, with 75 being strict anaerobes or microaerophilic species.
- Peptostreptococcus prevotii, Peptostreptococcus micros, and Fusobacterium necrophorum were the most frequently isolated strict anaerobes.
- Gram-positive anaerobic bacteria were found to be predominant in the root canal microbiota.
Conclusions:
- Gram-positive anaerobic bacteria are the dominant flora in root canals associated with periapical abscesses.
- Peptostreptococcus prevotii and Fusobacterium necrophorum demonstrated susceptibility to the evaluated antibiotics.