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Related Experiment Videos

The budding yeast Rad9 checkpoint complex: chaperone proteins are required for its function.

Christopher S Gilbert1, Michael van den Bosch, Catherine M Green

  • 1Cancer Research UK, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK.

EMBO Reports
|September 16, 2003
PubMed
Summary
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The study identifies Ssa1 and Ssa2 chaperone proteins as novel components of Rad9 complexes, crucial for DNA damage checkpoint regulation in Saccharomyces cerevisiae.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Genetics

Background:

  • Rad9 is a key protein in the DNA-damage checkpoint pathway in Saccharomyces cerevisiae.
  • Rad9 exists in large, soluble complexes that amplify checkpoint signals.
  • The precise composition and regulation of these complexes are not fully understood.

Purpose of the Study:

  • To purify and characterize the main soluble forms of Rad9 complexes involved in DNA damage response.
  • To investigate the role of associated chaperone proteins in Rad9 complex function and checkpoint activation.
  • To elucidate the functional contribution of Ssa1 and Ssa2 to DNA damage tolerance and cell cycle regulation.

Main Methods:

  • Purification of Rad9 complexes from whole-cell extracts of Saccharomyces cerevisiae.

Related Experiment Videos

  • Biochemical analysis to identify protein components of the purified complexes.
  • Genetic analysis to assess the roles of SSA1 and SSA2 in cellular responses to DNA damage.

    • Main Results:

    • Two major soluble forms of Rad9 complexes were purified to homogeneity.
    • Both purified Rad9 complexes contain Ssa1 and/or Ssa2 chaperone proteins.
    • Genetic experiments revealed redundant roles for SSA1 and SSA2 in cell survival, G2/M checkpoint control, and Rad9/Rad53 phosphorylation after UV irradiation.

    Conclusions:

    • Ssa1 and Ssa2 are novel checkpoint proteins involved in regulating Rad9 complexes.
    • These chaperones likely facilitate the remodeling of Rad9 complexes during checkpoint activation.
    • The findings provide new insights into the molecular mechanisms of DNA damage response pathways.