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Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
Claudia C Rubiano1, Moises Wasserman
1Laboratorio Bioquímica, Instituto Nacional de Salud, Bogotá, Colombia.
Memorias Do Instituto Oswaldo Cruz
|September 16, 2003
Abstract:
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.