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Planar chromatography: current status and future perspectives in pharmaceutical analysis--I. Applicability,
1Human Institute for Serobacterological Research and Production, Budapest, Hungary.
Journal of Pharmaceutical and Biomedical Analysis
|October 1, 1992
Summary
Capillary action planar chromatography offers accessible methods for pharmaceutical analysis. This review details its application, quantitation, and validation, highlighting evaluation techniques for optimal results.
Area of Science:
- Analytical Chemistry
- Chromatographic Techniques
Background:
- Capillary action planar chromatography is a versatile analytical method.
- Its operational simplicity and sensitivity to experimental conditions are key features.
- Selection and optimization of stationary and mobile phases are crucial for separation efficiency.
Purpose of the Study:
- To summarize the essential features of capillary action planar chromatographic methods.
- To detail the applicability, quantitation, and validation of these methods in pharmaceutical analysis.
- To discuss the advantages and limitations of various evaluation techniques.
Main Methods:
- Review of capillary action planar chromatographic principles.
- Discussion of phase system selection and optimization.
- Analysis of different detection and evaluation techniques (visual comparison, spot elution, in situ densitometry).
Main Results:
- Planar chromatography demonstrates significant applicability in pharmaceutical analysis.
- Detailed consideration of quantitation and validation specific to planar chromatography.
- Emphasis on validation steps unique to these chromatographic methods.
Conclusions:
- Capillary action planar chromatography provides effective solutions for pharmaceutical analysis.
- Understanding evaluation techniques and validation is critical for reliable results.
- The method's strengths and weaknesses are clearly outlined for practical application.