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Multidrug assay method for antimalarials.

R B Taylor1, R G Reid, R H Behrens

  • 1School of Pharmacy, Robert Gordon University, Aberdeen, UK.

Journal of Pharmaceutical and Biomedical Analysis
|October 1, 1992
PubMed
Summary
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A new method separates 11 antimalarial drugs and metabolites using solid-phase extraction and ion-pairing HPLC. This enables novel quantitative assays for malaria treatment and prophylaxis.

Area of Science:

  • Analytical Chemistry
  • Pharmacology

Background:

  • Antimalarial drug monitoring is crucial for effective malaria treatment and prophylaxis.
  • Existing separation and quantification methods may lack the specificity or efficiency required for complex biological matrices.

Purpose of the Study:

  • To develop a general separation strategy for a wide range of antimalarial drugs and their metabolites.
  • To establish a basis for novel quantitative assays relevant to malaria treatment and prophylaxis.

Main Methods:

  • Solid-phase extraction (SPE) for sample preparation.
  • Reversed-phase ion-pairing High-Performance Liquid Chromatography (RP-IP-HPLC) utilizing an organic counter ion for separation.
  • Development of quantitative assays based on the established separation technique.

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Main Results:

  • A robust separation strategy was successfully developed for 11 common antimalarial drugs and metabolites.
  • The underlying principles of the separation were explored, providing a foundation for method optimization.
  • Illustrative separations yielded quantitative data, demonstrating the potential for novel assay development.

Conclusions:

  • The developed SPE and RP-IP-HPLC method offers a versatile approach for analyzing antimalarial drugs and metabolites.
  • This strategy facilitates the creation of new quantitative assays crucial for malaria control efforts.
  • The findings are directly applicable to current practices in malaria prophylaxis and treatment.