Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell-free plasma layer in cerebral microvessels.

S Yamaguchi1, T Yamakawa, H Niimi

  • 1Department of Microcirculatory Science, National Cardiovascular Center Research Institute, Osaka, Japan.

Biorheology
|March 1, 1992
PubMed
Summary

The cell-free plasma layer thickness in feline cerebral microvessels depends on the pseudo shear rate, not just vessel diameter or red blood cell velocity. This layer thickens as the pseudo shear rate decreases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictive values of immune indicators on respiratory failure in the early phase of COVID-19 due to Delta and precedent variants.

Frontiers in immunology·2023
Same author

Recurrent deep vein thrombosis with a protein S Tokushima mutation.

The British journal of dermatology·2017
Same author

31 years of clinical hemorheology and microcirculation.

Clinical hemorheology and microcirculation·2012
Same author

Hyperactivation of the RAS signaling pathway in myelodysplastic syndrome with AML1/RUNX1 point mutations.

Leukemia·2006
Same author

Antiangiogenic activity of curcumin in hepatocellular carcinoma cells implanted nude mice.

Clinical hemorheology and microcirculation·2005
Same author

Quantitative assessment of cerebral neocapillary network and its remodeling in mice using intravital fluorescence videomicroscopy.

Angiogenesis·2003

Area of Science:

  • Physiology
  • Biophysics
  • Microcirculation Research

Background:

  • Understanding the cell-free plasma layer is crucial for comprehending blood flow dynamics in microvessels.
  • Cerebral microcirculation plays a vital role in brain function and is susceptible to changes in blood rheology.

Purpose of the Study:

  • To investigate the relationship between the cell-free plasma layer thickness and parameters like vessel diameter, red blood cell velocity, and pseudo shear rate in feline cerebral microvessels.
  • To determine the factors influencing the formation and dimensions of the cell-free plasma layer.

Main Methods:

  • Utilized a two fluorescent tracer method with fluorescein isothiocyanate (FITC)-labeled red blood cells to measure red cell velocity.
  • Employed rhodamine-B isothiocyanate (RITC)-labeled dextran to stain plasma for vessel diameter measurement.
  • Calculated red blood cell column diameter and vessel diameter to determine cell-free plasma layer thickness.

Main Results:

  • The thickness of the cell-free plasma layer was not solely dependent on vessel diameter or red blood cell velocity.
  • A significant correlation was found between cell-free layer thickness and the pseudo shear rate (cell velocity/vessel radius).
  • The cell-free plasma layer thickness increased with a decrease in the pseudo shear rate.

Conclusions:

  • The pseudo shear rate is a key determinant of cell-free plasma layer thickness in cerebral microvessels.
  • These findings provide insights into the rheological behavior of blood in the microvasculature.
  • Understanding these dynamics is important for studying conditions affecting cerebral blood flow.

Related Experiment Videos