[Simultaneous analysis of vitamin B complex by least square UV-spectrophotometry]

Simultaneous analysis of VitB1, VitB2 and VitB3 in Vitamin B Complex tablets was made by least square UV-spectrophotometry. The results showed that the contents of VitB1, VitB2 and VitB3 could be calculated by computer with least square method using 12 absorbances of 239, 241, 257, 259, 261, 263, 266, 269, 270, 279 and 281 nm wavelengths. The reclamation of VitB1 was 101.4% (CV% = 0.88, n = 3), of VitB2 98.5% (CV = 0.43, n = 3), and of VitB3 101.3% (CV% = 0.15, n = 3).