[Murine surgical model for the study of cultured grafts]

The objective of the study was to establish an animal model for in vivo studies of cultured cutaneous equivalents. The model on athymic mice that we already described (López-Valle C.A. et al., Plast Reconstr Surg, 1992, 89, 139-143) satisfied the criteria of immobilization of the recipient site and physical stability of the graft, but still allowing complete movement freedom of the animal used. Nevertheless, this technique encountered two long term weaknesses; a) a significant grafted surface reduction caused by the wound contraction and b) the absence of a physical barrier between human and murin keratinocytes. We propose some modifications to this technique to correct both problems. Moreover, the implantation of polypropylene, instead of glass pellets, to generate granulation tissue on the recipient bed when needed, constitutes an easier method for both the surgeon and the animal. Finally, in order to minimize wound care, some modifications were made to the rodent cages. Immunohistological analyses of the biopsies, 21 days post-grafting, revealed a continuous basal membrane. This animal model allows in vivo studies on the behavior, cicatrization and immunology of human cultured skin equivalents.