Related Experiment Videos
Specific allergen induced motility of peripheral blood mononuclear cells and polymorphonuclear leukocytes in insect
T Zak-Nejmark1, J Małolepszy, M Jutel
1Department of Internal Medicine and Allergology, Medical Academy, Wrocław, Poland.
Abstract:
The motility of peripheral blood mononuclear cells (MNC) and polymorphonuclear (PMN) leukocytes from normal and bee venom allergic subjects was investigated by a modified Boyden micropore filter method. The study comprised MNC locomotion in bee venom and histamine gradients and PMN locomotion in bee venom and fMLP gradients. We demonstrated statistically significant increase in MNC and PMN motility towards bee venom in allergic patients group. This effect disappeared after the preincubation of MNC with anti-human IgE antibodies. We observed no such effect in PMN leukocytes. Increased MNC motility in histamine gradient was observed only in control subjects group. Similarly significant increase in PMN locomotion towards fMLP was found in both allergic and control subjects. The results here demonstrated suggest that a specific allergen might be a chemoattractant for peripheral blood MNC and PMN leukocytes from atopics and could be capable to induce non-infectious inflammatory reactions as a result of its interaction with these sensitive cells.
Insights
Bee venom acts as a chemoattractant for immune cells in allergic individuals, increasing their motility. This allergen interaction may trigger inflammatory responses in sensitive patients.
Area of Science:
- Immunology
- Allergology
Background:
- Peripheral blood mononuclear cells (MNC) and polymorphonuclear (PMN) leukocytes play crucial roles in immune responses.
- Bee venom allergy is a significant health concern, necessitating a deeper understanding of its cellular mechanisms.
Purpose of the Study:
- To investigate the chemoattractant properties of bee venom on MNC and PMN leukocytes.
- To compare cellular motility in allergic and non-allergic individuals exposed to bee venom and other chemoattractants.
Main Methods:
- Modified Boyden micropore filter method was employed to assess cell motility.
- Mononuclear cells (MNC) were tested in bee venom and histamine gradients.
- Polymorphonuclear leukocytes (PMN) were tested in bee venom and fMLP gradients.
Main Results:
- A statistically significant increase in MNC and PMN motility towards bee venom was observed in allergic subjects.
- Preincubation of MNC with anti-human IgE antibodies abolished the increased motility towards bee venom.
- Increased MNC motility in histamine gradients was specific to control subjects.
- PMN locomotion towards fMLP increased in both allergic and control groups.
Conclusions:
- Specific allergens, like bee venom, can act as chemoattractants for peripheral blood MNC and PMN leukocytes in atopic individuals.
- Allergen-cell interaction may induce non-infectious inflammatory reactions.
- The observed effects on MNC motility are likely mediated by IgE antibodies.