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Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between
S B Zotchev1, L I Soldatova, A V Orekhov
1Institute of Genetics, Moscow, Russia.
Research in Microbiology
|November 1, 1992
Abstract:
Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3'- and 5'-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains.