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A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
Published on: January 17, 2018
A method for thin sectioning heavily sclerotized cuticle of Odontotaenius disjunctus
J W Sawvel1, S Gunderson, R C Schiavone
1Dept. of Biological Sciences, Ohio Northern University, Ada 45810.
Abstract:
Elytra of the bessbeetle, Odontotaenius disjunctus were thin sectioned after embedding in epoxy resin. Sections were cut with a diamond saw, ground to the desired thickness on a rotary grinder and polished. Tearing and distortion were reduced when compared to knifecut sections of heavily sclerotized cuticle.

