Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Definition of a consensus binding site for p53.

W S el-Deiry1, S E Kern, J A Pietenpol

  • 1Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231.

Nature Genetics
|April 1, 1992
PubMed
Summary

Researchers identified the specific DNA sequences that the tumor suppressor protein p53 binds to. This discovery is crucial for understanding how p53 functions in preventing cancer.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum to "Annotated normal CT data of the abdomen for deep learning: Challenges and strategies for implementation" [Diagn. Interv. Imaging. 101 (2020) 35-44].

Diagnostic and interventional imaging·2020
Same author

Differentiating autoimmune pancreatitis from pancreatic ductal adenocarcinoma with CT radiomics features.

Diagnostic and interventional imaging·2020
Same author

Deep lessons learned: Radiology, oncology, pathology, and computer science experts unite around artificial intelligence to strive for earlier pancreatic cancer diagnosis.

Diagnostic and interventional imaging·2019
Same author

Annotated normal CT data of the abdomen for deep learning: Challenges and strategies for implementation.

Diagnostic and interventional imaging·2019
Same author

Circulating tumor DNA as a potential marker of adjuvant chemotherapy benefit following surgery for localized pancreatic cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

Circulating tumor DNA as an early marker of therapeutic response in patients with metastatic colorectal cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2015

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • The tumor suppressor protein p53 plays a critical role in cellular responses to DNA damage.
  • Previous studies suggested that p53's function is linked to its interaction with DNA.

Purpose of the Study:

  • To identify and characterize the specific DNA sequences that bind to the p53 protein.
  • To understand the structural requirements of these DNA binding sites for p53 interaction.

Main Methods:

  • Screening of human genomic clones for p53 binding using in vitro assays.
  • Precise mapping of p53 binding sequences within identified genomic clones.
  • Analysis of p53 mutant binding affinities to defined DNA motifs.

Main Results:

Related Experiment Videos

  • Identification of 18 human genomic clones that bind to p53 in vitro.
  • Discovery of a consensus binding site characterized by internal symmetry, comprising two copies of a specific 10-base pair motif.
  • Demonstration that a single motif copy is insufficient for binding, and alterations in the motif abolish p53 affinity.
  • Observation that p53 mutants, including those frequently altered in cancer, do not bind to the consensus dimer.

Conclusions:

  • The study defines the precise DNA sequence elements essential for p53 interaction in vitro.
  • These findings provide insights into the in vivo mechanisms of p53 action and its role in cancer suppression.