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Glucose biosensor using glucose oxidase immobilized in polyaniline
A H Parente1, E T Marques, W M Azevedo
1Departamento de Bioquimica, Laboratorio de Imunopatologia, Keizo Asami-LIKA, Brazil.
Applied Biochemistry and Biotechnology
|December 11, 1992
Summary
A novel glucose biosensor was developed using immobilized glucose oxidase on polyaniline. This optical sensor demonstrates a linear response and stability, showing promise for clinical applications.
Area of Science:
- Biomedical Engineering
- Electrochemistry
- Enzyme Kinetics
Background:
- Glucose oxidase (EC 1.1.3.4.) is crucial for glucose detection.
- Polyaniline offers a promising matrix for enzyme immobilization.
- Optical sensing methods are valuable for clinical diagnostics.
Purpose of the Study:
- To develop a glucose biosensor utilizing glucose oxidase immobilized on polyaniline.
- To characterize the kinetic properties and stability of the immobilized enzyme.
- To explore the potential of polyaniline-based optical sensors for glucose monitoring.
Main Methods:
- Enzyme immobilization via covalent bonding of glucose oxidase onto polyaniline using glutaraldehyde.
- Characterization of sensor response to glucose concentration.
- Assessment of enzyme activity at different pH levels.
- Evaluation of sensor stability over time.
- Analysis of optical absorption spectra changes.
Main Results:
- A linear response to glucose was observed up to 2.2 mM with a response time of 2.5-4.0 min.
- Optimal enzyme activity was found between pH 6.5 and 7.5.
- The biosensor retained 50% of its activity after 30 days of daily use.
- Significant changes in optical absorption spectra around 800 nm were detected upon glucose interaction.
Conclusions:
- The developed polyaniline/glucose oxidase biosensor exhibits favorable performance characteristics.
- The observed optical variation indicates polyaniline's suitability as a support for optical glucose sensors.
- This system holds potential for clinical applications in glucose monitoring.