Effect of tooth storage and cavity cleansing on cervical gap formation in Class II glass-ionomer/composite...

Class II cavities with the cervical margin just below the C-E junction and with two cervical retention grooves were prepared in intact human premolars, 31 cavities in teeth stored frozen and 29 cavities in teeth stored in 1% chloramine solution. The teeth from both storage conditions were assigned to two groups; in one group, the cavities were cleaned with a water spray, in the other group, with a cleaner containing 0.2% EDTA and 1% NaF. All cavities were lined with Vitrebond and restored with P50. Cervical contraction gaps were disclosed by a fluorescent resin penetration technique. The length and width of each gap were analysed. A contraction gap was revealed in 17 of the 31 cavities in teeth stored frozen (54.8%) and in 22 of the 29 restorations in the teeth stored in chloramine (75.8%). The width of the gap was significantly greater in teeth stored in chloramine (p = 0.0004). No statistical difference in gap formation was found between cavities cleaned with water or with the detergent containing 0.2% EDTA and sodium fluoride. Since the cavity cleansing procedure was not a significant factor in gap formation, apparently removing the superficial smear layer with the cavity cleaner does not impair the bonding of Vitrebond to the dentin. However, gap formation appeared to be significantly affected by the storage conditions of the extracted teeth. It is suggested that teeth to be used in bond strength and leakage experiments should be stored frozen rather than stored in 1% chloramine solution.