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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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A novel method for blood group typing with the aid of monoclonal antibody immobilized dyed microspheres

S Yazawa1, H Ohkawara, T Nakajima

  • 1Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.

Nihon Hoigaku Zasshi = the Japanese Journal of Legal Medicine
|December 1, 1992
PubMed

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...