In vitro influence of oxygenation on lipogenesis of rat adipose tissue

Pre-incubation of rat adipose tissue from ad lib fed rats in gas mixtures containing a range of oxygen concentrations from 0% oxygen to 20% oxygen showed that incorporation of isotopes of glucose into carbon dioxide and tissue were increased after pre-incubation for a period of 14-16 hours in a gas atmosphere containing 5% oxygen. This increase required the presence of 1.2 U/ml insulin in the pre-incubation medium and it was augmented by the presence of 2.8 x 10(-7) M prostaglandin F2 alpha. Incorporation into tissue was shown to be mainly into glyceride-glycerol. Similar results were obtained with isolated fat cells.